ON THE CONDITIONS NEEDED TO VERIFY A NONSTATIONARY DIFFUSION-MODEL BYKINETIC-ANALYSIS OF FAST FLUORESCENCE QUENCHING .2.

The performance of distinguishing fluorescence quenching kinetics base d on the Smoluchowski-Collins-Kimball (SCK) treatment from those showi ng single-exponential decays for donors with lifetimes lower than 1 ns is examined, Experimental instrument response functions with FWHM = 3 5 ps and FWHM = 400 ps have been used to generate the decays. The resu lts of these numerical experiments can be used by experimentalists to better plan experiments and to ensure the parameters, which they obtai n from fitting fluorescence decay curves in the presence of quencher, are valid and not interdependent.