A MONOCLONAL-ANTIBODY, DL10, WHICH RECOGNIZES A SUGAR MOIETY OF MHC CLASS-I ANTIGENS EXPRESSED ON NK CELLS, NK-CELLS, AND GRANULOCYTES IN HUMANS( T)

One mAb, DL10, was established from mice injected with dolphin lymphoc ytes. In addition to its reactivity against all dolphin lymphocytes, i t reacted with some human leukocytes, including NK cells, NK+ T cells, and granulocytes. When its reactivity was examined in various animals , bovine, ovine, and equine leukocytes were DL10(+). Murine, rat, and canine leukocytes were DL10(-). Although the reactivity of DL10(+) was similar to those of CD56 and CD57 antigens in humans, the actual mole cules it recognized were different. Thus, all reactivity of DL10 disap peared after treatment of cells with glycopeptidase or after culture o f cells with tunicamycin. Furthermore, the immunoprecipitation method revealed that DL10 indirectly recognized the heavy chain (45kD) of MHC class I antigen in humans and animals. Considering data from analysis of the N-terminal amino acid sequence of the DL10 molecule and the HL A typing of reactive cells, DL10 recognized a sugar moiety of some mon omorphic MHC antigens and polymorphic MHC antigens such as KLA-B60 and -B61. If the donors are HLA-B60(-) and -B61(-)(> 80% in Japan and > 9 5% in the United States), DL10 would appear to be a very useful agent for the detection of pan-NK+ T cells.