MATURE SENSORY NEURON-DERIVED HYBRID CELL-LINE EXPRESSING NGF-DEPENDENT NEURITE EXTENSION

Authors
HIKAWA N TAKENAKA T
Citation
N. Hikawa et T. Takenaka, MATURE SENSORY NEURON-DERIVED HYBRID CELL-LINE EXPRESSING NGF-DEPENDENT NEURITE EXTENSION, Brain research, 774(1-2), 1997, pp. 225-228
Citations number
18
Journal title
Brain research → ACNP
ISSN journal
00068993
Volume
774
Issue
1-2
Year of publication
1997
Pages
225 - 228
Database
ISI
SICI code
0006-8993(1997)774:1-2<225:MSNHCE>2.0.ZU;2-G
Abstract
We established a hybrid cell line which extended neurites in the prese nce of nerve growth factor by fusion between adult mouse sensory neuro ns and neuroblastoma C1300 cells using emetine and actinomycin D. The serine-phosphorylated 200 and 160 kDa neurofilament proteins were dete cted in the hybrid cells. In comparison, C1300 cells expressed both su bunit of non-phosphorylated neurofilaments at serine residues. (C) 199 7 Elsevier Science B.V.