THE PROLIFERATIVE RESPONSE OF HIV-CELLS (CD4+ AND CD8+) ARE SEVERELY SUPPRESSED VIA CD28 COACTIVATION( T)

Several studies have suggested that regulation of expression of the co stimulatory molecule CD28 on the T-cell surface may play an important role in AIDS pathogenesis. In a study of T-cells from HIV+ donors, we find that activation with anti-CD3 plus anti-CD28 results in a mitogen ic response which was approximately 86% suppressed for both CD4+ and C D8+ T-cells when compared to normal control cells. With PMA costimulat ion (instead of anti-CD28), the anti-CD3 response was suppressed much less, by 64 and 61%, respectively. With Con A as opposed to CD3 stimul ation, the degree of suppression was less with either coactivator but still more severe with CD28 than with PMA coactivation. It has been re ported that the CD28 subset of CD8+ T-cells is diminished in HIV+ indi viduals and could account for these results. It is possible as well th at the CD28 costimulatory pathway in the CD4+ T-cells particularly is altered due to intervention by the HIV. While our data do not differen tiate between these two possibilities, it show that the immune status is compromised in the HIV+ individual not only in terms of number of C D4+ T-cells, but in their activation response as well.