MAST-CELL-MEDIATED INDUCTION OF ICAM-1, VCAM-1 AND E-SELECTIN IN ENDOTHELIAL-CELLS IN-VITRO - CONSTITUTIVE RELEASE OF INDUCING MEDIATORS BUT NO EFFECT OF DEGRANULATION
Cmcj. Vanhaaster et al., MAST-CELL-MEDIATED INDUCTION OF ICAM-1, VCAM-1 AND E-SELECTIN IN ENDOTHELIAL-CELLS IN-VITRO - CONSTITUTIVE RELEASE OF INDUCING MEDIATORS BUT NO EFFECT OF DEGRANULATION, Pflugers Archiv, 435(1), 1997, pp. 137-144
Mast cell (MC)-mediated induction of intercellular adhesion molecule-l
(ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) and of E-sele
ctin was studied in cultures of rat heart endothelial cells (EC) and h
uman umbilical vein EC (HUVEC) respectively. MC induced VCAM-1 and E-s
electin, but hardly any ICAM-1 in EC. Induction was not dependent on M
C degranulation, but seemed to be provoked by constitutively released
substances, other than histamine, from MC. Coincubation of MC and EC,
allowing for direct contact between the two cell types, was more poten
t in induction than MC co-incubated separately from EC using a permeab
le membrane. MC were less potent in induction than exogenous added cyt
okines or LPS. Induction of cell adhesion molecules in rat heart EC wa
s MC-specific, since EC incubations with either rat cardiomyocytes or
heart fibroblasts had no effect. The data show that rat MC, independen
t of degranulation, secrete mediators relevant for the induction of a
specific set of EC adhesion molecules in vitro. This suggests a (suppo
rtive) role for MC in cell-adhesion molecule induction in the endothel
ium in settings of early or mild inflammation. The results are discuss
ed in the context of inflammatory processes in the heart in vivo.