TCRV AND TCRJ GENE USAGE IN MBP RESPONDING T-CELLS FROM (B10.PL X PL J)F1 MICE IS BIASED TOWARDS THAT OF B10.PL MICE/

We analyzed myelin basic protein (MBP) specific T cell hybridoma clone s from (B10.PL X PL/J)Fl mice. MBP-reacting T cell hybridomas from Fl mice preferentially expressed B10.PL TcraV2.3 (53%) and B10.PL TcraV4. 2 (13%) with minor expression of TcraV4.4 (13%) gene segments. A domin ant expression of TcrbV8.2 (73%) accompanying with TcrbV8.1 (20%) and TcrbV13 (7%) gene segments have been identified in these MBP-reacting T cell hybridomas from Fl mice. There was less restrictive but non-ran dom usage of the TcraJ and TcrbJ gene segments. Overall, the MBP-react ing T cell hybridomas from (B10.PL X PL/J)Fl mice were dominated by th e MBP-reacting T cell pattern seen in B10.PL mice. (C) 1997 Elsevier S cience B.V.