CLONING, SEQUENCING, GENE ORGANIZATION, AND LOCALIZATION OF THE HUMANRIBOSOMAL-PROTEIN RPL23A GENE

The intron-containing gene for human ribosomal protein RPL23A has been cloned, sequenced, and localized, The gene is approximately 4.0 kb in length and contains five exons and four introns. All splice sites exa ctly match the AG/GT consensus rule. The transcript is about 0.6 kb an d is detected in all tissues examined. In adult tissues, the RPL23A tr anscript is dramatically more abundant in pancreas, skeletal muscle, a nd heart, while much less abundant in kidney, brain, placenta, lung, a nd liver. A full-length cDNA clone of 576 nt was identified, and the n ucleotide sequence was found to match the exon sequence precisely, The open reading frame encodes a polypeptide of 156 amino acids, which is absolutely conserved with the rat RPL23A protein. In the 5' flanking region of the gene, a canonical TATA sequence and a defined CAAT box w ere found for the first time in a mammalian ribosomal protein gene. Th e intron-containing RPL23A gene was mapped to cytogenetic band 17q11 b y fluorescence in situ hybridization. (C) 1997 Academic Press.