Md. Fernandeztome et al., ENDOGENOUS PROSTAGLANDINS REGULATE RAT RENAL PHOSPHOLIPID DE-NOVO SYNTHESIS, Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1349(1), 1997, pp. 55-66
Rat renal papilla is the zone of the kidney enjoying the most active p
hospholipid metabolism and also the highest prostanlandin production.
We studied the phospholipid biosynthesis and the relationship between
phospholipid de novo synthesis and prostaglandin biosynthesis in rat r
enal papilla. Indomethacin inhibited the biosynthesis of phosphatidylc
holine, phosphatidylinositol, phosphatidylethanolamine and phosphatidi
c acid. Exogenous PGF(2 alpha) and PGD(2) restored biosynthetic activi
ty in the presence of indomethacin and also increased the activity of
the enzymes involved in the Kennedy pathway. The decrease in phospholi
pid biosynthesis maintained a linear relationship with the decrease in
prostaglandin biosynthesis. Moreover, esculetin, which stimulates pro
staglandin synthesis, brought about a significant increase in P-32 inc
orporation to the three phospholipids studied. The evidence presented
in this paper indicates that renal PGF(1 alpha) and PGD(2) modulate ph
ospholipid de novo synthesis in rat renal papilla. (C) 1997 Elsevier S
cience B.V.