THE EFFECTS OF TEMPERATURE AND CHORIONIC-GONADOTROPIN ON THE FUNCTIONAL-PROPERTIES OF LACTATE-DEHYDROGENASE FROM OOCYTES OF LOACH MISGURNUS-FOSSILIS

Acclimation of leach to relatively low and high temperatures gives ris e to changes in the properties of LDH from oocytes, The minimum of app arent K-m values for pyruvate of LDH from immature oocytes of fish acc limated to low temperature was registered at low assay temperatures, w hereas adaptation to high temperatures leads to the enzyme showing a m inimum at high temperatures. In thermal acclimation of fish the K-m mi nimum value drifts during 15 days, During oocyte maturation, the K-m m inimum drifts during 40 hr, The differences in the kinetic features of LDH from oocytes both at thermal acclimation and during oocyte matura tion are not associated with the appearance of new isozymes.