A DIRECT ELECTRODE-DRIVEN P450 CYCLE FOR BIOCATALYSIS

Authors
REIPA V MAYHEW MP VILKER VL
Citation
V. Reipa et al., A DIRECT ELECTRODE-DRIVEN P450 CYCLE FOR BIOCATALYSIS, Proceedings of the National Academy of Sciences of the United Statesof America, 94(25), 1997, pp. 13554-13558
Citations number
26
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
94
Issue
25
Year of publication
1997
Pages
13554 - 13558
Database
ISI
SICI code
0027-8424(1997)94:25<13554:ADEPCF>2.0.ZU;2-7
Abstract
The large potential of redox enzymes to carry out formation of high va lue organic compounds motivates tile search for innovative strategies to regenerate the cofactors needed by their biocatalytic cycles. Here, we describe a bioreactor where the reducing power to the cycle is sup plied directly to purified cytochrome CYP101 (P450cam; EC 1.14.15.1) t hrough its natural redox partner (putidaredoxin) using an antimony-dop ed tin oxide working electrode, Required oxygen was produced at a Pt c ounter electrode by water electrolysis. A continuous catalytic cycle w as sustained for more than 5 h and 2,600 enzyme turnovers. The maximum product formation rate was 36 nmol of 5-exo-hydroxycamphor/nmol of CY P101 per min.