Sleep curtailment constitutes an increasingly common condition in indu
strialized societies and is thought to affect mood and performance rat
her than physiological functions. There is no evidence for prolonged o
r delayed effects of sleep loss on the hypothalamo-pituitary-adrenal (
HPA) axis. We evaluated the effects of acute partial or total sleep de
privation on the nighttime and daytime profile of cortisol levels. Pla
sma cortisol profiles were determined during a 32-hour period (from 18
00 hours on day 1 until 0200 hours on day 3) in normal young men submi
tted to three different protocols: normal sleep schedule (2300-0700 ho
urs), partial sleep deprivation (0400-0800 hours), and total sleep dep
rivation. Alterations in cortisol levels could only be demonstrated in
the evening following the night of sleep deprivation. After normal sl
eep, plasma cortisol levels over the 1800-2300-hour period were simila
r on days 1 and 2. After partial and total sleep deprivation, plasma c
ortisol levels over the 1800-2300-hour period were higher on day 2 tha
n on day 1 (37 and 45% increases, p = 0.03 and 0.003, respectively), a
nd the onset of the quiescent period of cortisol secretion was delayed
by at least 1 hour. We conclude that even partial acute sleep loss de
lays the recovery of the HPA from early morning circadian stimulation
and is thus likely to involve an alteration in negative glucocorticoid
feedback regulation. Sleep loss could thus affect the resiliency of t
he stress response and may accelerate the development of metabolic and
cognitive consequences of glucocorticoid excess.