M. Atmaca et al., EVALUATION OF A COMMERCIAL KIT FOR GLUTATHIONE ESTIMATION IN CELLULAR-SYSTEMS, ATLA. Alternatives to laboratory animals, 25(6), 1997, pp. 667-673
The major aim of this study was to identify an appropriate assay to us
e routinely for cellular glutathione measurement. The Saville assay ha
s been widely used in cytotoxicity studies, while the GSH-400 assay is
a commercial kit which only recently became available. Therefore, in
this study, the accuracy and sensitivity of the Saville and GSH-400 as
says were compared. Results presented herein indicate that the Saville
assay gave a lower blank absorbance and higher sensitivity when compa
red to the GSH-400 assay.