EFFECTS OF ANTIHYPERTENSIVE DRUGS ON ANTIOXIDANT ENZYME-ACTIVITIES AND RENAL-FUNCTION IN STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS

Citation
I. Shou et al., EFFECTS OF ANTIHYPERTENSIVE DRUGS ON ANTIOXIDANT ENZYME-ACTIVITIES AND RENAL-FUNCTION IN STROKE-PRONE SPONTANEOUSLY HYPERTENSIVE RATS, The American journal of the medical sciences, 314(6), 1997, pp. 377-384
Citations number
38
ISSN journal
00029629
Volume
314
Issue
6
Year of publication
1997
Pages
377 - 384
Database
ISI
SICI code
0002-9629(1997)314:6<377:EOADOA>2.0.ZU;2-N
Abstract
The reactive oxygen species has been proposed as a key mediator of the progression of renal injury associated with essential hypertension. A mong the defense systems operating against the reactive oxygen species , superoxide dismutase, glutathione peroxidase, and catalase are the m ost important antioxidant enzymes (AOEs). In the present study, systol ic blood pressure, renal function (creatinine clearance, urinary album in, and N-acetyl-beta D-glucosaminidase excretion), renal intrinsic AO E activities, and renal histopathology were determined in stroke-prone spontaneously hypertensive rats and Wistar Kyoto rats. The effects of a 20-week treatment using three antihypertensive drug regimens-captop ril, a sulfhydryl-containing angiotensin-converting enzyme inhibitor; temocapril, a potent, non-sulfhydryl-containing angiotensin-converting enzyme inhibitor prodrug; and a conventional triple drug combination that includes a vasodilator (hydralazine, hydrochlorothiazide and rese rpine)-on renal function, renal tissue, AOE activities, and renal hist opathologic abnormalities were evaluated in stroke-prone spontaneously hypertensive rats. Renal function and AOE activities were lower in th e stroke-prone spontaneously hypertensive rats than in the Wistar Kyot o rats. Normalization of systolic blood pressure using the antihyperte nsive drugs improved renal function and produced a nonuniform alterati on in renal AOEs; only glutathione peroxidase activity increased signi ficantly with the use of all three drug regimens. The mild renal histo pathologic abnormality in stroke-prone spontaneously hypertensive rats was not altered by drug treatment. The improvement in renal function may be related to an increase in glutathione peroxidase activity, but no correlation was seen between renal function changes and alteration in activities of superoxide dismutase or catalase.