The activity of soil ureases was evaluated in the laboratory in soils
from three locations in the Serengeti National Park, Tanzania, differi
ng in the grazing intensities that the grasslands there support. Ureas
e activity was assayed by the instantaneous release of NH4 as soon as
soils drained to held capacity after application of an aqueous urea so
lution approximating N concentrations in ungulate urine. The appearanc
e of NO3 and SO4 in extracts was used as an index of biological activi
ty and pH changes; neither responded to urea addition. Ammonium appear
ance in extracts of soils to which water but not urea was applied was
low and identical; appearance in extracts where urea had been added wa
s high and differed between sites, increasing with the level of grazer
activity at a site. The data document ecologically meaningful levels
of soil urease in Serengeti soils and a positive association of those
levels with grazing intensity.