W. Maleewong et al., DETECTION OF PARAGONIMUS-HETEROTREMUS IN EXPERIMENTALLY INFECTED CAT FECES BY ANTIGEN CAPTURE ELISA AND BY DNA HYBRIDIZATION, The Journal of parasitology, 83(6), 1997, pp. 1075-1078
An antigen capture enzyme-linked immunosorbent assay (antigen capture-
ELISA) and DNA hybridization technique were developed and evaluated fo
r their application in the detection of Paragonimus heterotremus infec
tion in experimentally infected cars. An IgG fraction prepared from se
rum of a rabbit immunized with P. heterotremus excretory-secretory (ES
) products was used as the capture antibody. An IgG, monoclonal antibo
dy specific to the 22- and 31.5-kDa ES products of P. heterotremus was
used as the antigen probe. As little as 0.24 ng of the ES products co
uld be detected by this technique. A specific P. heterotremus DNA prob
e derived from the P. heterotremus genomic DNA library containing 1,50
0 base pairs was used in a dot-blot hybridization assay for the detect
ion of parasite DNA. The radioactively labeled probe could detect DNA
released from as few as 2 P. heterotremus eggs. Both ELISA and DNA hyb
ridization were found to have 100% specificity, with sensitivities of
73.7% and 100%, respectively.