Ak. Volety et Fle. Chu, ACID-PHOSPHATASE-ACTIVITY IN PERKINSUS-MARINUS, THE PROTISTAN PARASITE OF THE AMERICAN OYSTER, CRASSOSTREA-VIRGINICA, The Journal of parasitology, 83(6), 1997, pp. 1093-1098
The effect of temperature (4, 12, 20, and 28 C) and osmolality (400, 5
70, and 840 mOsm/kg) on extracellular acid phosphatase (AP) secretion
in vitro, and ultrastructural localization of AP activity in the paras
ite were investigated. The extracellular AP secretion by Perkinsus mar
inus was cell density dependent (P < 0.001). Increasing culture temper
atures resulted in increased P. marinus proliferation concomitant with
AP secretion (P < 0.0001). AP secretion was similar in P. marinus cul
tured at 400 and 570 mOsm/kg media, but higher than P. marinus culture
d at 870 mOsm/kg media. Results of the ultrastructural study revealed
that intense AP activity was in the nucleus of the parasite. Based on
its distribution in the nucleus, AP may be playing a role in events le
ading to cell cycle regulation.