BIOLIGAND INTERACTION ASSAY BY FLOW-INJECTION ABSORPTIOMETRY

A novel technique for the study of bioligand interactions based on com bining non; injection on renewable surfaces with UV-visible absorptiom etry is introduced. The concept is proven by monitoring the binding of various proteins to protein G and the binding of various insulin anal ogs to a monoclonal anti-insulin antibody, thus providing a comparison with the performance of surface plasmon resonance (SPR)-based techniq ues. The advantages of the bioligand interaction now injection absorpt iometry approach are speed, low cost, no need for regeneration of soli d substrate, and spectral resolution not available with SPR sensing, I t is believed that this technique will have an impact on the entire bi osensor held since it allows simultaneous monitoring of labeled as web as nonlabeled species in real time over a wide spectral range with hi gh sensitivity.