The action of microorganisms is generally dependent on factors such as
availability of organic or inorganic nutrients, sunlight, and moistur
e. In this study, a combination of factors occurring during manufactur
ing and storage were found to cause mold growth on cotton swabs. These
factors included improper or inadequate sanitization, contaminated Me
thocel, inadequate amounts of preservative (0.1% or less) in the infes
ted Methocel, high moisture levels (30% or more) in cotton swabs, plas
tic sticks/put ups, and warehousing cotton swabs under hot and humid c
onditions. Sealed plastic bags used for storage, essentially unventila
ted units, were found to provide an ideal moisture chamber for mildew
development. Implementation of certain control options solved the abov
e problems, ensuring mildew-free manufacturing of cotton swabs.