SITE-DIRECTED DISULFIDE MAPPING OF HELICES M4 AND M6 IN THE CA2-TWITCH SKELETAL-MUSCLE SARCOPLASMIC-RETICULUM( BINDING DOMAIN OF SERCA1A, THE CA2+ ATPASE OF FAST)

In an attempt to define the spatial relationships among SERCA1a transm embrane helices M4, M5, M6, and M8, involved in Ca2+ binding, all six cysteine residues were removed from predicted transmembrane sequences by substitution with Ser or Ala. The cysteine-depleted protein retaine d 44% of wild type Ca2+ transport activity. Pairs of cysteine residues were then reintroduced to determine whether their juxtaposition would result in the formation of disulfide cross-links between transmembran e helices. In initial studies de signed to map the juxtaposition of Ca 2+ binding residues, Cys was substituted for Glu(309) or Gly(310) in t ransmembrane sequence M4, in combination with the substitution of Cys for Glu(771) in M5; for Asn(796), Thr(789), or Asp(800) in M6; or for Glu(908) in M8. These double mutants all retained the capacity to form a phosphoenzyme intermediate from P-i (but not from ATP in the presen ce of Ca2+), and in all but mutants E309C/N796C and G310C/N796C, phosp hoenzyme formation was insensitive to 100 mu M Ca2+. These results sup port the view that both Glu(309) and Asn(796) contribute to Ca2+ bindi ng site II, which is not required for conversion of E-2, the substrate for P-i phosphorylation, to E-1. Cross linking in mutants E309C/N796C and G310C/D800C established reference points for the orientation of M 4 and M6 relative to each other and provided the basis for the predict ion of potential additional cross-links. Strong links were formed with the pairs T317C/A804C and T317C/L807C near the cytoplasmic ends of th e two helices and with A305C/L792C and A305C/L793C near the lumenal en ds. These combined results support the conclusion that M4 and M6 form a right-handed coiled-coil structure that forms part of the pathway of Ca2+ translocation. In addition to providing a possible explanation f or the mutation sensitivity of several pairs of residues in these heli ces, the proposed association of M4 and M6 supports a new model for th e orientation of the two Ca2+ binding sites among transmembrane helice s M4, M5, and M6.