CONTROL OF HUMAN LUTEAL STEROIDOGENESIS - ROLE OF GROWTH HORMONE-RELEASING HORMONE, VASOACTIVE-INTESTINAL-PEPTIDE, AND PITUITARY ADENYLATE CYCLASE-ACTIVATING PEPTIDE
R. Apa et al., CONTROL OF HUMAN LUTEAL STEROIDOGENESIS - ROLE OF GROWTH HORMONE-RELEASING HORMONE, VASOACTIVE-INTESTINAL-PEPTIDE, AND PITUITARY ADENYLATE CYCLASE-ACTIVATING PEPTIDE, Fertility and sterility, 68(6), 1997, pp. 1097-1102
Objective: To examine the possible effect of growth hormone-releasing
hormone (GHRH), vasoactive intestinal peptide, and pituitary adenylate
cyclase-activating peptide on basal and hCG-stimulated P production b
y human luteal cells. Design: Cultures of human luteal cells from the
early and midluteal phase. Setting: All corpora lutea were obtained fr
om the Obstetrics and Gynecology Department of the Universita Cattolic
a, a public care center. Patient(s): Ten nonpregnant women between 35
and 47 years of age underwent surgery for various nonendocrine disorde
rs, such as leiomyomatosis. Intervention(s): Corpora lutea were obtain
ed at the time of hysterectomy. Main Outcome Measure(s): Luteal cells
were incubated with GHRH, vasoactive intestinal peptide, and pituitary
adenylate cyclase-activating peptide with or without hCG at different
concentrations. Result(s): Pituitary adenylate cyclase-activating pep
tide stimulated P production in a dose- and time-dependent manner, whe
reas GHRH and vasoactive intestinal peptide did not affect luteal ster
oidogenesis. None of the three peptides were found to synergize with h
CG. Conclusion(s): Pituitary adenylate cyclase-activating peptide can
influence human luteal steroidogenesis. (C) 1997 by American Society f
or Reproductive Medicine.