THE INFLUENCE OF METABOLITES ON ENZYME-BASED FLOW-INJECTION ANALYSIS

The influence of metabolites, as ethanol, L-lactate, pyruvate, acetate , glycerol and succinate, in concentrations, which are typical for Esc herichia coli cultivation, on the enzyme based flow injection analysis was investigated. All of these effects are reversible. Alcohol oxidas e, glucose oxidase, lactate dehydrogenase, lactate monooxigenase and s arcosine oxidase, immobilized on VA-Epoxy-Biosynth E3 carrier, were us ed for the analysis of ethanol, glucose, pyruvate, L-lactate and aceta te, respectively. The immobilized enzymes were filled in small cartrid ges and integrated into flow injection analysis systems. The particula r metabolites and their combination were used in the concentration ran ge of 0-8 g l(-1). Alcohol oxidase and glucose oxidase are strongly im paired by these metabolites, lactate monooxigenase and lactate dehydro genase are less sensitive to them, and sarcosine oxidase is only sligh tly influenced by the presence of these metabolites. (C) 1997 Elsevier Science B.V.