M. Shigyo et al., ASSIGNMENT OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS TO ALL CHROMOSOMES OF SHALLOT (ALLIUM-CEPA L. AGGREGATUM GROUP), Genes & genetic systems, 72(4), 1997, pp. 249-252
The chromosomal locations of randomly amplified polymorphic DNA (RAPD)
markers were examined in shallot (Allium cepa L. Aggregatum group). A
series of alien monosomic addition lines of Japanese bunching onion (
A. fistulosum L.) with extra chromosomes (1A-8A) from A. cepa Aggregat
um group was used as plant materials. Several decamer (OPERON KITs A,
E, and G) and 12-mer (Wake DNA Oligomer sets A-1, C-4, F-4, and F-5) o
ligonucleotides were applied as random primers. The chromosomal locati
ons of the 16 RAPD markers were determined; the RAPD marker OPE09(1600
) was located on the chromosome 1A of A. cepa Aggregatum group, OPE03(
600) and OPE18(1400) on 2A, OPA12(700) on 3A, OPA11(950), OPE18(1500),
OPG10(800), WAA09(1800), WAF65(900), and WAF83(800) on 4A, OPE17(500)
and OPE18(2000) on 5A, OPG08(500) on 6A, WAF81(2800) and WAF83(2200)
on 7A, and WAC68(500) on 8A. Consequently, the RAPD markers were assig
ned to all the chromosomes of A. cepa Aggregatum group.