ASSIGNMENT OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS TO ALL CHROMOSOMES OF SHALLOT (ALLIUM-CEPA L. AGGREGATUM GROUP)

Citation
M. Shigyo et al., ASSIGNMENT OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS TO ALL CHROMOSOMES OF SHALLOT (ALLIUM-CEPA L. AGGREGATUM GROUP), Genes & genetic systems, 72(4), 1997, pp. 249-252
Citations number
15
Journal title
ISSN journal
13417568
Volume
72
Issue
4
Year of publication
1997
Pages
249 - 252
Database
ISI
SICI code
1341-7568(1997)72:4<249:AORAPD>2.0.ZU;2-I
Abstract
The chromosomal locations of randomly amplified polymorphic DNA (RAPD) markers were examined in shallot (Allium cepa L. Aggregatum group). A series of alien monosomic addition lines of Japanese bunching onion ( A. fistulosum L.) with extra chromosomes (1A-8A) from A. cepa Aggregat um group was used as plant materials. Several decamer (OPERON KITs A, E, and G) and 12-mer (Wake DNA Oligomer sets A-1, C-4, F-4, and F-5) o ligonucleotides were applied as random primers. The chromosomal locati ons of the 16 RAPD markers were determined; the RAPD marker OPE09(1600 ) was located on the chromosome 1A of A. cepa Aggregatum group, OPE03( 600) and OPE18(1400) on 2A, OPA12(700) on 3A, OPA11(950), OPE18(1500), OPG10(800), WAA09(1800), WAF65(900), and WAF83(800) on 4A, OPE17(500) and OPE18(2000) on 5A, OPG08(500) on 6A, WAF81(2800) and WAF83(2200) on 7A, and WAC68(500) on 8A. Consequently, the RAPD markers were assig ned to all the chromosomes of A. cepa Aggregatum group.