IMMUNOLOGICAL CHARACTERIZATION OF HETEROCHROMATIN PROTEIN P25-BETA AUTOANTIBODIES AND RELATIONSHIP WITH CENTROMERE AUTOANTIBODIES AND PULMONARY FIBROSIS IN SYSTEMIC SCLERODERMA

Anticentromere antibodies (ACA) are immunological markers for the subs et of systemic scleroderma with the symptoms calcinosis cutis, Raynaud 's phenomenon, esophageal dysfunction, sclerodactyly, and telangiectas ia (CREST). In western blotting, some ACA-positive sera also recognize a doublet of 23 kDa (p23) and 25 kDa (p25) in addition to centromere protein antigens A (17 kDa), B (80 kDa), and C (140 kDa). Two forms of p25 have been shown to be human homologues of Drosophila heterochroma tin-associated protein HP1. One form of p25 (p25 beta) which was recen tly cloned in this laboratory was used to evaluate anti-p25 beta antib ody response in scleroderma sera. Of 318 scleroderma sera 42 had ACA ( 13.2%), and 16 of the 42 sera (38%) had anti-p25 beta antibodies. On t he other hand, 5 of 276 ACA-negative sera (1.8%) showed anti-p25 beta antibody response, demonstrating that anti-p25 beta antibody is signif icantly associated with the ACA response (P<10(-8)). Clinically the an ti-p25 beta response was significantly associated with the CREST syndr ome. Fourteen (36.8%) of 38 CREST patients compared to seven (2.5%) of 280 patients with other forms of scleroderma were anti-p25 beta antib ody pos itive (P<10(-8)). The 14 CREST patients with anti-p25 beta ant ibodies had significantly more interstitial lung disease than those wi thout anti-p25 beta antibodies (P<0.003). There was also a tendency to increased liver involvement. Two dominant autoepitopes in p25 beta we re determined by western blotting using p25 beta recombinant fragments . In immunofluorescence C-terminal specific antibodies showed staining of heterochromatin, but N-terminal specific antibodies showed no stai ning. Interestingly, the majority of sera reacted preferentially with one or the other of the two dominant autoepitopes.