EFFECT OF DIFFERENT PORE DIAMETER SILICA-BASED POLYMER-BONDED PHASES AND MOBILE-PHASE ON SEPARATION OF PROTEINS

The preparation of silica matrices of different pore diameter, the syn thesis of different pore diameter silica-based polymer-bonded phases a nd their chromatographic characteristics in the separation of proteins are described. The results obtained show that macroporous packings ha ve a relatively small surface area and low capacity, and are therefore , suitable for the quick separation and analysis of biopolymer. The se paration mechanism changes as the pore diameter increases and the mobi le phase is altered. The effect of acetonitrile concentration on the r etention of proteins on a 10 nm pore diameter silica-based polymer-bon ded phase is discussed, together with the effect of salt concentration and pH value of the eluent on the retention of proteins on 300 nm por e diameter silica-based polymer-bonded phases.