THE IRRADIATION OF HEPATOCYTES WITH HE-NE-LASER CAUSES AN INCREASE OFCYTOSOLIC-FREE CALCIUM-CONCENTRATION AND AN INCREASE OF CELL-MEMBRANEPOTENTIAL, CORRELATED WITH IT, BOTH INCREASES TAKING PLACE IN AN OSCILLATORY MANNER

Authors
VACCA RA MORO L PETRAGALLO VA GRECO M FONTANA F PASSARELLA S
Citation
Ra. Vacca et al., THE IRRADIATION OF HEPATOCYTES WITH HE-NE-LASER CAUSES AN INCREASE OFCYTOSOLIC-FREE CALCIUM-CONCENTRATION AND AN INCREASE OF CELL-MEMBRANEPOTENTIAL, CORRELATED WITH IT, BOTH INCREASES TAKING PLACE IN AN OSCILLATORY MANNER, Biochemistry and molecular biology international, 43(5), 1997, pp. 1005-1014
Citations number
21
Journal title
Biochemistry and molecular biology international → ACNP
ISSN journal
10399712
Volume
43
Issue
5
Year of publication
1997
Pages
1005 - 1014
Database
ISI
SICI code
1039-9712(1997)43:5<1005:TIOHWH>2.0.ZU;2-F
Abstract
Isolated hepatocytes were irradiated with Helium-Neon laser (fluence: 0.24 Joules x cm(-2) fluence rate: 12 mW x cm(-2)) and changes of both cytosolic free Ca2+ concentration and cell membrane potential were ch ecked by measuring fura-2 and bis-oxonol fluorescence respectively. Ir radiation resulted in an enhancement in cytosolic free Ca2+ concentrat ion that requires the presence of Ca2+ in the phase outside hepatocyte s; consistently an increase in cell membrane potential was measured co rrelated with it. Interestingly, the rate of increase of both cytosoli c free Ca2+ concentration and cell membrane potential shows special ti me dependent features similar to those peculiar of oscillatory process es.