KINETIC-ANALYSIS OF THE FENTON REACTION BY ESR-SPIN TRAPPING

A quantitative analysis of hydroxyl radical (. OH) generated in the Fe 2+-hydrogen peroxide reaction system was explored by a spin-trapping m ethod using-5,5-himethyl-1-pyrroline-N-oxide (DMPO) combined with elec tron spin resonance spectroscopy. Based on the numerical analysis of F enton-related reactions, the reduction of DMPO-OH adduct from 1:1 stoi chiometry prominent at high concentrations of Fe2+ was consistent with a reaction model in which a molar amount of hydrogen peroxide was red uced by two molar amounts of Fe2+ Furthermore, lime-dependent decrease in DMPO-OH quantity, apparent at much higher concentration of Fe2+, w as proved due to reaction not with Fe2+ but with Fe3+.