CIRCULATING LEVELS OF LOCUSTA DIURETIC HORMONE - THE EFFECT OF FEEDING

Authors
AUDSLEY N GOLDSWORTHY GJ COAST GM
Citation
N. Audsley et al., CIRCULATING LEVELS OF LOCUSTA DIURETIC HORMONE - THE EFFECT OF FEEDING, Peptides, 18(1), 1997, pp. 59-65
Citations number
22
Categorie Soggetti
Biology
Journal title
Peptides → ACNP
ISSN journal
01969781
Volume
18
Issue
1
Year of publication
1997
Pages
59 - 65
Database
ISI
SICI code
0196-9781(1997)18:1<59:CLOLDH>2.0.ZU;2-Y
Abstract
A radioimmunoassay was developed using I-125-labeled- [Tyr(0)]Locusta- DH and polyclonal antibodies raised against Locusta-DH(29-46). The ass ay had a detection limit of 50 pM, and displayed limited crossreactivi ty for other CRF-related peptides, but not for unrelated peptides. Abo ut 60% of the total immunoreactive material in locust hemolymph was at tributable to Locusta-DH. The circulating level of diuretic hormone in creases fivefold in fed insects, sufficient to stimulate primary urine production, and is correlated with the duration of the meal. This is consistent with the role of Locusta-DH in the control of postfeeding d iuresis. (C) 1997 Elsevier Science Inc.