Hh. Woo et Mc. Hawes, CLONING OF GENES WHOSE EXPRESSION IS CORRELATED WITH MITOSIS AND LOCALIZED IN DIVIDING CELLS IN ROOT CAPS OF PISUM-SATIVUM L, Plant molecular biology, 35(6), 1997, pp. 1045-1051
Removal of border cells from pea roots synchronizes and induces root c
ap cell division, wall biogenesis and differentiation. Three messages
which are expressed differentially in such induced root caps have been
cloned. Sequence analyses showed that the PsHRGP1-encoded protein has
high homology with a hydroxyproline-rich glycoprotein. The PsCaP23-en
coded protein has high homology with an alfalfa callus protein or tran
slationally controlled human or mouse tumor protein P23. The PsRbL41-e
ncoded protein has high homology with a highly basic 60S ribosomal pro
tein L41. in situ hybridization showed that PsHRGP1, PsCaP23 and PsRbL
41 messages are localized within dividing cells of the root cap. PsHRG
P1 is highly expressed in uninduced root caps, but its message is repr
essed by 10-11 times as soon as cell division and differentiation begi
n. Expression of PsHRGP1 recovers to higher than (180%) its initial le
vel in 30 min. PsHRGP1 is root-specific. PsCaP23 and PsRbL41 messages
increase ca. 3-fold within 15 min after root cap induction. All three
genes represent small families of 3-5 closely related genes in the pea
genome.