DETERMINATION OF ORGANIC AND INORGANIC ANIONS IN INSECT HEMOLYMPH ANDMIDGUT CONTENTS BY ION CHROMATOGRAPHY

A simple and rapid ion-exchange chromatographic method with conductime tric detection for the determination of carboxylic acids (succinic, ma lic and citric) and inorganic anions (chloride, phosphate and sulphate ) in haemolymph and midgut content of Lepidoptera larvae is provided. The mobile phase is 0.975 mM phthalic acid at pH 4.1. The procedure of sample extractions was simple. Comparison of the results with whose r eported in the bibliography showed that the values were similar. The i on-exchange chromatographic-conductimetric detection method permits th e analysis of various organic and inorganic anions in small biological samples. (C) 1997 Elsevier Science B.V.