ACTIVITY OF LYSOSOMAL SYSTEM IN MOUSE-LIVER AFTER TAXOL ADMINISTRATION

1. The effect of taxol on selected lysosomal enzymes (cathepsin D, lys osomal lipase, beta-glucuronidase, beta-glucosidase, alanine aminopept idase) in mouse hepatocytes after 24 hr treatment by increasing doses (0.75 mg/kg bw, 1.25 mg/kg bw and 2.5 mg/kg bw) was studied. 2. The se gments were also taken from the mice for ultrastructural studies with the use of electron microscopy. The greatest changes in activity of en zymes at the taxol dose of 2.5 mg/kg bw were as follows: the activity of cathepsin D increased by 71%, that of alanine aminopeptidase increa sed by 103%, that of beta-glucuronidase decreased by 45% and that of b eta-glucosidase decreased by 63%. 3. The significant changes observed in the hepatocyte ultrastructure were closely correlated with biochemi cal changes that were dependent on the taxol dosage. (C) 1998 Elsevier Science Inc.