HIGH-RESOLUTION HLA-DQB1 TYPING USING THE POLYMERASE CHAIN-REACTION AND SEQUENCE-SPECIFIC PRIMERS

Authors
MULLIGHAN CG BUNCE M WELSH KI
Citation
Cg. Mullighan et al., HIGH-RESOLUTION HLA-DQB1 TYPING USING THE POLYMERASE CHAIN-REACTION AND SEQUENCE-SPECIFIC PRIMERS, Tissue antigens, 50(6), 1997, pp. 688-692
Citations number
25
Journal title
Tissue antigens → ACNP
ISSN journal
00012815
Volume
50
Issue
6
Year of publication
1997
Pages
688 - 692
Database
ISI
SICI code
0001-2815(1997)50:6<688:HHTUTP>2.0.ZU;2-2
Abstract
Polymorphism at HLA-DQB1 is known to influence tissue compatibility an d disease susceptibility; however, current DQB1 typing methods are una ble to distinguish the 32 currently recognized DQB1 alleles. We have d eveloped a 32-reaction PCR-SSP method capable of differentiating all D QB1 alleles that differ in amino acid sequence. This method can resolv e all heterozygous combinations of DQB1 alleles, with the exception of several combinations involving alleles not thus far detected in Cauca soid populations.