EVALUATION OF QUAIL EGG-WHITE RIBOFLAVIN BINDING-PROTEIN AS A CHIRAL SELECTOR IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS

A new chiral stationary phase for high-performance liquid chromatograp hy of quail egg white riboflavin binding protein is presented. Several chiral acidic, basic and uncharged drugs were analysed and the influe nce of the mobile phase's parameters on the retention times and enanti oselectivity was evaluated. On the basis of the results obtained, the same protein was studied as a background electrolyte additive in free solution capillary electrophoresis, in order to evaluate if capillary electrophoresis (CE) could be used as a rapid scouting technique for s creening the enantioselectivity of novel proteins without immobilisati on on a solid support. To investigate if it is possible to directly co mpare the results obtained by each technique, the CE experiments were planned on the basis of both the findings and ideas originated in liqu id chromatography. (C) 1997 Elsevier Science B.V.