EFFECTS OF ADVANCED GLYCATION END-PRODUCTS ON THE PROLIFERATION AND DIFFERENTIATION OF OSTEOBLAST-LIKE CELLS

Two different lines of osteoblast-like cells were used to investigate the effect of advanced glycation end-products of bovine serum albumin on cell proliferation and differentiation. These parameters were found to be both dose- and time-dependent. Cell proliferation remained unch anged after a 24 h incubation period, it increased after intermediate periods of incubation with advanced glycation end-products, but was fo und to be depressed after several days incubation. Cellular alkaline p hosphatase activity followed a similar pattern: an initial increase in duced by advanced glycation end-products was generally followed, after relatively long incubation periods, by a slight but significant decre ase in this parameter. Ca-45(2+) uptake was only significantly inhibit ed by advanced glycation end-products after 24 h incubation. These res ults suggest that advanced glycation endproducts directly regulate ost eoblast proliferation and differentiation in a dose and time dependent manner.