Kv. Penmetsa et al., ENANTIOMERIC AND ISOMERIC SEPARATION OF HERBICIDES USING CYCLODEXTRIN-MODIFIED CAPILLARY-ZONE-ELECTROPHORESIS, Journal of chromatography, 790(1-2), 1997, pp. 225-234
Cyclodextrin-modified capillary zone electrophoresis (CD-CZE) was appl
ied successfully to the enantiomeric and isomeric separation of three
herbicides (imazaquin, diclofop and imazamethabenz). Commercially avai
lable cyclodextrins were evaluated for separation of the enantiomers a
nd isomers of the three herbicides having Varied molecular structures.
The enantiomers of imazaquin and diclofop, and the isomers of imazame
thabenz could be resolved with a resolution of greater than or equal t
o 1.5. The resolution was found to depend on pH of the run buffer, cyc
lodextrin type and cyclodextrin concentration. By employing mixed cycl
odextrins in the running buffer, the three herbicides were simultaneou
sly separated in a single run. In addition, rapid (less than 3 min) en
antiomeric separation is demonstrated using imazaquin as a model herbi
cide. The reported capillary electrophoresis (CE) methods are simple,
rapid, efficient and reproducible and our results demonstrate that CE
provides a powerful analytical tool for enantiomeric and isomeric sepa
ration of herbicides. (C) 1997 Elsevier Science B.V.