CONTRIBUTION OF DUAL STAINING FOR CD13 CD 14 MARKERS IN COMBINATION WITH CD34 DETERMINATION FOR PERIPHERAL-BLOOD STEM-CELL COLLECTION/

We evaluated the reliability of a flow cytometry technique for countin g mononuclear cells (MNCs) in cytapheresis products. Eighty freshly-pr epared samples of peripheral stem cells were studied using a dual immu nolabeling technique with antibodies to CD13/CD14, and were also label ed with anti-CD34. Results of this immunophenotype determination were compared to those of the conventional method for counting MNCs under t he microscope. Dual CD13/CD14 labeling was found to be a simple and re liable method for counting MNCs in the presence of immature and stimul ated cells. When used in combination with CD34 labeling, the dual immu nolabeling method helped improve the evaluation of the quality of peri pheral stem cell grafts.