MEDIA SUPPLEMENTATION TO SUPPORT GROWTH OF HELICOBACTER-PYLORI

Growth of Helicobacter pylori is better on solid medium than in liquid medium. This investigation studied the growth of H. pylori in Brain H eart Infusion Broth (BHI) with 10% Fetal Calf (FCS) only, 10% FCS + 0. 5 unit/mL Oxyrase(TM) and 0.5 unit/mL Oxyrase(TM incubated microaerobi cally and aerobically. High populations (ca. 5.5 log CFU/mL) could be detected in aerobic incubation in broth when Oxyrase(TM) was added. Ho wever, in BHI media without Oxyrase(TM) after incubation for 24 h aero bically the numbers of H. pylori decreased from log 7.3 CFU/mL to none detectable. BHI with 10% FCS and BHI were adequate liquid enrichment media for the growth of H. pylori under microaerophilic conditions.