A 2-HOUR, SINGLE-STEP RT-PCR PROTOCOL FOR DETECTION OF RNA-VIRUS

Authors
WANG RF CAMPBELL W CERNIGLIA CE
Citation
Rf. Wang et al., A 2-HOUR, SINGLE-STEP RT-PCR PROTOCOL FOR DETECTION OF RNA-VIRUS, Journal of rapid methods and automation in microbiology, 5(4), 1997, pp. 321-324
Citations number
8
Journal title
Journal of rapid methods and automation in microbiology → ACNP
ISSN journal
10603999
Volume
5
Issue
4
Year of publication
1997
Pages
321 - 324
Database
ISI
SICI code
1060-3999(1997)5:4<321:A2SRPF>2.0.ZU;2-G
Abstract
A rapid protocol of RT-PCR for the detection of RNA-Virus, Mouse Hepat itis Virus, was developed. An Idaho Air-Thermal Cycler war used with 5 mM MgCl2 in the buffer so that both RT and PCR steps can be run in on e tube and the total detection time is less than two hours.