DETERMINATION OF LYSERGIDE IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COMBINED WITH ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY

A quantitative method which avoids derivatisation is described for the determination of lysergide (LSD) levels in urine. Sample preparation included addition of methysergide as an internal standard followed by solid-phase extraction. LSD was analysed on a system consisting of a C -18 stationary phase and a mobile phase of 0.1 M acetate buffer pH 8.0 -acetonitriletriethylamine (75:25:0.25, v/v). LSD was detected by elec trospray ionisation mass spectrometry with selected ion monitoring. Th e quantification limit was 0.5 ng/ml and the method was linear up to 1 0 ng/ml of LSD in urine.