CAPILLARY ELECTROPHORESIS OF METHOTREXATE POLYGLUTAMATES AND ITS APPLICATION IN EVALUATION OF GAMMA-GLUTAMYL HYDROLASE ACTIVITY

Authors
WALTHAM MC LIN SL LI WW GOKER E GRITSMAN H TONG WP BERTINO JR
Citation
Mc. Waltham et al., CAPILLARY ELECTROPHORESIS OF METHOTREXATE POLYGLUTAMATES AND ITS APPLICATION IN EVALUATION OF GAMMA-GLUTAMYL HYDROLASE ACTIVITY, Journal of chromatography B. Biomedical sciences and applications, 689(2), 1997, pp. 387-392
Citations number
17
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
689
Issue
2
Year of publication
1997
Pages
387 - 392
Database
ISI
SICI code
0378-4347(1997)689:2<387:CEOMPA>2.0.ZU;2-A
Abstract
A rapid and sensitive procedure for the separation of methotrexate (MT X) polyglutamates(2) using capillary electrophoresis (CE) is described as it applies to the in vitro assay of the enzyme gamma-glutamyl hydr olase (GGH, EC 3.4.22.12). Distinct separation of MTX and polyglutamyl ated forms (up to glu(4)) is achieved within 10 min using a 75 mu m I. D. capillary (50 cm, +25 kV), and enables quantitation of both reactan t and enzyme products. As activity can be reliably determined using le ss than 5X10(5) eukaryotic cells, this new technique can be used to me asure GGH in patient tumor samples and investigate the relationship be tween GGH levels and clinical MTX resistance.