SIMULTANEOUS DETERMINATION OF A NOVEL CALCIUM-ENTRY BLOCKER, MONATEPIL MALEATE, AND ITS METABOLITES IN RAT PLASMA BY MEANS OF SOLID-PHASE EXTRACTION AND REVERSED-PHASE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

Authors
KURONO M SUZUKI K YOSHIDA K NARUTO S
Citation
M. Kurono et al., SIMULTANEOUS DETERMINATION OF A NOVEL CALCIUM-ENTRY BLOCKER, MONATEPIL MALEATE, AND ITS METABOLITES IN RAT PLASMA BY MEANS OF SOLID-PHASE EXTRACTION AND REVERSED-PHASE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION, Journal of chromatography B. Biomedical sciences and applications, 689(2), 1997, pp. 427-432
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
689
Issue
2
Year of publication
1997
Pages
427 - 432
Database
ISI
SICI code
0378-4347(1997)689:2<427:SDOANC>2.0.ZU;2-9
Abstract
A reversed-phase LC method with electrochemical detection is described for the simultaneous determination of monatepil maleate (AJ-2615, AJ) , a novel calcium entry blocker, and its three S-oxidized metabolites in plasma. These compounds were extracted from plasma by solid-phase e xtraction and injected onto an ODS column. The determination limit in plasma (0.5 ml) was 10 ng/ml for AJ and 5 ng/ml for the three metaboli tes. The method was applied to the determination of AJ and the metabol ites in rat plasma samples.