DETECTION OF GENETICALLY-MODIFIED INSECT-RESISTANT BT MAIZE BY MEANS OF POLYMERASE CHAIN-REACTION

Authors
HUPFER C HOTZEL H SACHSE K ENGEL KH
Citation
C. Hupfer et al., DETECTION OF GENETICALLY-MODIFIED INSECT-RESISTANT BT MAIZE BY MEANS OF POLYMERASE CHAIN-REACTION, ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY, 205(6), 1997, pp. 442-445
Citations number
16
Journal title
ZEITSCHRIFT FUR LEBENSMITTEL-UNTERSUCHUNG UND-FORSCHUNG A-FOOD RESEARCH AND TECHNOLOGY → ACNP
ISSN journal
14314630
Volume
205
Issue
6
Year of publication
1997
Pages
442 - 445
Database
ISI
SICI code
1431-4630(1997)205:6<442:DOGIBM>2.0.ZU;2-C
Abstract
A method for the detection of genetically modified insect-resistant ma ize expressing a synthetic gene encoding a truncated version of the Cr yIA(b) protein derived from Bacillus thuringiensis is described. The p rocedure includes: (I) extraction of genomic DNA, (2) amplification of the inserted synthetic gene bq means of the polymerase chain reaction (PCR) method, and (3) characterization and confirmation of the PCR pr oduct by Southern hybridization with a digoxigenin-labelled oligo-nucl eotide probe and DNA sequencing.