SOLID-PHASE EXTRACTION AND DETERMINATION OF RANITIDINE IN HUMAN PLASMA BY A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD UTILIZING MIDBORE CHROMATOGRAPHY

Authors
FARTHING D BROUWER KLR FAKHRY I SICA D
Citation
D. Farthing et al., SOLID-PHASE EXTRACTION AND DETERMINATION OF RANITIDINE IN HUMAN PLASMA BY A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD UTILIZING MIDBORE CHROMATOGRAPHY, Journal of chromatography B. Biomedical sciences and applications, 688(2), 1997, pp. 350-353
Citations number
8
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
688
Issue
2
Year of publication
1997
Pages
350 - 353
Database
ISI
SICI code
0378-4347(1997)688:2<350:SEADOR>2.0.ZU;2-2
Abstract
An improved high-performance liquid chromatographic (HPLC) method util izing solid-phase extraction (SPE) and midbore chromatography was deve loped for the determination of ranitidine in human plasma. A mobile ph ase of 20 mM K2HPO4-acetonitrile-triethylamine (87.9:12.0:0.1, v/v) pH 6.0 was used with a phenyl analytical column and ultraviolet detectio n (UV). The method demonstrated linearity from 25 to 1000 ng/ml in 500 mu l of plasma with a detection limit of 10 ng/ml. The method was uti lized in a pharmacokinetic study evaluating the effects of pancreatico -biliary secretions on ranitidine absorption.