HYDROPHOBIC CHROMATOGRAPHY OF THE HL-60 CELLULAR-FRACTION CO-BINDING WITH HEXAMETHYLENE BISACETAMIDE

Methods of separating N-acetyl-1,6-diaminohexane (NADAH) and its immob ilization to diol-silica have been developed. Hexamethylene bisacetami de (HMBA) and its metabolite NADAH are used as inducers of leukemia ce ll differentiation. The inducing mechanism of HMBA is still not clear. Experiments show that HMBA and NADAH undergo relatively strong hydrop hobic reactions and do not readily undergo ion-exchange with the prote ins of the cytosolic fraction of HL-60 cells during immobilization of NADAH; the retention time of the proteins was longer than that of the phosphatides. These results show that the adsorption of HMBA and NADAH to proteins was higher than that to phosphatides. The expected biospe cific receptor binding with HMBA has not been found.