A NOVEL, NEGATIVE SELECTABLE MARKER FOR GENE DISRUPTION IN DICTYOSTELIUM

Authors
MORRISON A MARSCHALEK R DINGERMANN T HARWOOD AJ
Citation
A. Morrison et al., A NOVEL, NEGATIVE SELECTABLE MARKER FOR GENE DISRUPTION IN DICTYOSTELIUM, Gene, 202(1-2), 1997, pp. 171-176
Citations number
28
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
202
Issue
1-2
Year of publication
1997
Pages
171 - 176
Database
ISI
SICI code
0378-1119(1997)202:1-2<171:ANNSMF>2.0.ZU;2-A
Abstract
The expression of an ochre suppressor mutant of the (II)-I-Glu(UUA) tR NA appears to be lethal to Dictyostelium, and offers a novel 'positive -negative' strategy to select for targeted gene disruption by homologo us recombination. Inclusion of the suppressor tRNA gene decreases the overall transformation frequency by approximately 20-fold. This increa ses the proportion of targeted gene disruptions to over 90%. (C) 1997 Elsevier Science B.V.