COMPARISON OF ATB STAPH, RAPID ATB STAPH, VITEK, AND E-TEST METHODS FOR DETECTION OF OXACILLIN HETERORESISTANCE IN STAPHYLOCOCCI POSSESSINGMECA

The performance characteristics of the E-test (AB Biodisk, Solna, Swed en), the ATE Staph, the Rapid ATE Staph, and the Vitek GPS-503 card (b ioMerieux, La Balme Les Grottes, France) methods for the detection of oxacillin resistance in a collection of staphylococci with a high prop ortion of troublesome strains were evaluated. Sixty-four Staphylococcu s aureus strains and 76 coagulase-negative staphylococcal strains were tested. All strains were mecA positive and were characterized by the oxacillin agar screen plate test; 75 (53.6%) were found to be heteroge neous by a large-inoculum oxacillin disk diffusion assay, and oxacilli n MICs for 89 (63.6%) were less than or equal to 32 mu g/ml. Three (4. 7%) S. aureus strains and 25 (32.9%) coagulase-negative strains were c lassified as susceptible by the E-test, as defined by the National Com mittee for Clinical Laboratory Standards (NCCLS) oxacillin breakpoint (MIG less than or equal to 2 mu g/ml). The ATE Staph method failed to detect oxacillin resistance in 7 (11%) S. aureus isolates and 32 (42.1 %) coagulase-negative isolates. The MICs for all but six of these disc repant isolates were less than or equal to 16 mu g/ml. The Rapid ATE S taph method was tested against S. aureus strains only and yielded 15 ( 23.4%) false-susceptible results for strains for which the MICs were l ess than or equal to 32 mu g/ml. The Vitek system was the best-perform ing system, since it failed to detect oxacillin resistance in only 3 ( 4.7%) S. aureus strains and 15 (19.7%) coagulase-negative strains, the MICs for all of which were less than or equal to 2 mu g/ml. These dat a indicate that (i) the performance of the two ATE Staph systems can b e limited when the prevalence of borderline-heteroresistant staphyloco cci is high and (ii) the unreliability of the E-test and the Vitek met hods for detecting resistant coagulase-negative strains might be reduc ed by the potential revision of the oxacillin breakpoint currently rec ommended by the NCCLS.