INHIBITION OF PCR BY AGAR FROM BACTERIOLOGICAL TRANSPORT MEDIA

Authors
Citation
Ap. Gibb et S. Wong, INHIBITION OF PCR BY AGAR FROM BACTERIOLOGICAL TRANSPORT MEDIA, Journal of clinical microbiology, 36(1), 1998, pp. 275-276
Citations number
7
Categorie Soggetti
Microbiology
ISSN journal
00951137
Volume
36
Issue
1
Year of publication
1998
Pages
275 - 276
Database
ISI
SICI code
0095-1137(1998)36:1<275:IOPBAF>2.0.ZU;2-9
Abstract
We observed inhibition of PCR in throat swabs submitted in routine bac teriological transport media. Experimental studies showed that agar, w hich was extracted,vith DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent. No inhibitory effect was observed with a tra nsport medium which did not contain agar.