AMPEROMETRIC BIOSENSORS FOR L-ALANINE AND PYRUVATE ASSAYS IN BIOLOGICAL-FLUIDS

Authors
GILIS M DURLIAT H COMTAT M
Citation
M. Gilis et al., AMPEROMETRIC BIOSENSORS FOR L-ALANINE AND PYRUVATE ASSAYS IN BIOLOGICAL-FLUIDS, Analytica chimica acta, 355(2-3), 1997, pp. 235-240
Citations number
18
Journal title
Analytica chimica acta → ACNP
ISSN journal
00032670
Volume
355
Issue
2-3
Year of publication
1997
Pages
235 - 240
Database
ISI
SICI code
0003-2670(1997)355:2-3<235:ABFLAP>2.0.ZU;2-3
Abstract
Two amperometric electrochemical biosensors have been developed and us ed to determine the concentrations of L-alanine and pyruvate in biolog ical fluids (blood, fermentation media) without any prior treatment of the sample. The proposed biosensors have response times lower than 5 min, a precision of 2% and the measured current is proportional to the substrate concentration in the range from 1 x 10(-6) to 2 x 10(-3) mo l l(-1). They have an operating life of one month. (C) 1997 Elsevier S cience B.V.