INCREASE OF SENSITIVITY AND VALIDITY OF THE SOS UMU-TEST AFTER REPLACEMENT OF THE BETA-GALACTOSIDASE REPORTER GENE WITH LUCIFERASE/

Authors
SCHMID C REIFFERSCHEID G ZAHN RK BACHMANN M
Citation
C. Schmid et al., INCREASE OF SENSITIVITY AND VALIDITY OF THE SOS UMU-TEST AFTER REPLACEMENT OF THE BETA-GALACTOSIDASE REPORTER GENE WITH LUCIFERASE/, Mutation research. Genetic toxicology and environmental mutagenesis, 394(1-3), 1997, pp. 9-16
Citations number
17
Journal title
Mutation research. Genetic toxicology and environmental mutagenesis → ACNP
ISSN journal
13835718
Volume
394
Issue
1-3
Year of publication
1997
Pages
9 - 16
Database
ISI
SICI code
1383-5718(1997)394:1-3<9:IOSAVO>2.0.ZU;2-C
Abstract
The SOS/umu-test with Salmonella TA1535/pSK1002 as tester strain is a rapid and valuable bacterial assay for screening of umuC-dependent mut agenic potential of chemical compounds and chemicals relevant to envir onmental pollution. The initial assay was modified by replacing the be ta-galactosidase reporter gene with luciferase. Thereby, the sensitivi ty of the until-test was increased significantly and the susceptibilit y to intensively coloured solutions was reduced. The alternative enzym e assay in the modified umu-test (umu-Luc) represents an independent m ethod which allows to confirm the colorimetric results obtained with t he original SOS/umu-test system (umu-Gal) by measuring the beta-galact osidase activity. It has been proved that for several genotoxins an in creased induction rate could be achieved by applying the new umu-Luc t est. (C) 1997 Elsevier Science B.V.