THE VICINAL CHLOROALCOHOLS 1,3-DICHLORO-2-PROPANOL (DC2P), 3-CHLORO-1,2-PROPANEDIOL (3CPD) AND 2-CHLORO-1,3-PROPANEDIOL (2CPD) ARE NOT GENOTOXIC IN-VIVO IN THE WING SPOT-TEST OF DROSOPHILA-MELANOGASTER
H. Frei et Fe. Wurgler, THE VICINAL CHLOROALCOHOLS 1,3-DICHLORO-2-PROPANOL (DC2P), 3-CHLORO-1,2-PROPANEDIOL (3CPD) AND 2-CHLORO-1,3-PROPANEDIOL (2CPD) ARE NOT GENOTOXIC IN-VIVO IN THE WING SPOT-TEST OF DROSOPHILA-MELANOGASTER, Mutation research. Genetic toxicology and environmental mutagenesis, 394(1-3), 1997, pp. 59-68
In this study, the vicinal chloroalcohols 1,3-dichloro-2-propanol (DC2
P), 3-chloro-1,2-propanediol (3CPD) and 2-chloro-1,3-propanediol (2CPD
) were investigated for genotoxicity in the wing spot test of Drosophi
la. DC2P is an important starting material in many processes of synthe
sis in chemical industry. 3CPD as well as some related glycerol chloro
hydrins were identified in protein hydrolysates industrially used for
the production of food items such as seasonings, sauces and soups. The
wing spot test is a somatic mutation and recombination test (SMART) a
nd is a sensitive in vivo assay for the detection of mutagens and prom
utagens. The test was applied here in its standard version with normal
bioactivation and in a variant with increased cytochrome P450-depende
nt bioactivation capacity. All three compounds were clearly non-genoto
xic in these in vivo assays. The results are in agreement with recent
findings which strongly suggest that positive genotoxicity results in
in vitro testing of vicinal chloroalcohols such as DC2P are due to dir
ectly acting genotoxic intermediates arising from a chemical reaction
with the culture medium rather than from enzymatic biotransformation.
(C) 1997 Elsevier Science B.V.