DERIVATIZATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PENTAAZAPENTACOSANE PENTAHYDROCHLORIDE

Authors
HEIMBECHER S LEE YC TABIBI SE YALKOWSKY SH
Citation
S. Heimbecher et al., DERIVATIZATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF PENTAAZAPENTACOSANE PENTAHYDROCHLORIDE, Journal of chromatography B. Biomedical sciences and applications, 691(1), 1997, pp. 173-178
Citations number
15
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications → ACNP
ISSN journal
13872273
Volume
691
Issue
1
Year of publication
1997
Pages
173 - 178
Database
ISI
SICI code
0378-4347(1997)691:1<173:DAHLAO>2.0.ZU;2-J
Abstract
A rapid high-performance liquid chromatographic method for determinati on of the dansyl derivative of pentaazapentacosane (PAPC) pentahydroch loride has been developed. The chromatographic system uses a reversed- phase C-8 column, a mobile phase of acetic acid buffer and acetonitril e and UV detection. The dansylation conditions were optimized with a p H of 11.0 and a 20-fold dansyl chloride excess. The yield of dansyl PA PC increased 10-fold as the reaction pH was changed from 9.5 to 10.5. Under derivatization conditions of pH 8.5-11.0 and 1-30-fold excess da nsyl chloride only perdansyl PAPC was found.